Journal: REPRODUCTION
Article Title: Involvement of VCAM1 in the bovine conceptus adhesion to the uterine endometrium
doi: 10.1530/rep-13-0655
Figure Lengend Snippet: Figure 5 Expression of VCAM1 receptor, integrin a 4 (ITGA4), by bovine conceptuses. (A) Changes in ITGA4 mRNAs in days 17, 20, and 22 conceptuses (P17, P20, and P22 respectively). Note that ITGA4 transcripts were found minimal at the uterine epithelium and stroma. Values represent meanGS.E.M. from three independent samples with duplicates within a day of conceptus collection. **Statistically significant differences in mRNA levels (P!0.01). (B) Immunohisto- chemical analysis of ITGA4 (a, b, c, and d) or VCAM1 (e, f, g, and h) expression in the bovine uterus obtained from day 22 pregnant animals. Tissue sections (10 mm) from day 22 uteri were immunostained for ITGA4 using an anti-ITGA4 antibody (a and c) or normal mouse IgG as a negative control at low (b) and higher magnification (d). (c) Observation of boxed area in B-a at a higher magnification. Tissue sections were immunostained for VCAM1 using an anti-VCAM1 antibody (e and g), or normal rabbit IgG as a negative control at low (f) and higher magnification (h). (g) Observation of boxed area in B-e at a higher magnification. Epi, endometrial luminal epithelium; St, endometrial stroma; Tr, trophoblast. Black scale barZ200 mm and white scale barZ40 mm.
Article Snippet: After 30 min of incubation with 10% normal goat serum, the sections were incubated at 4 8C overnight with a rabbit anti-human VCAM1 polyclonal antibody (1:100 dilution, 0.5 mg/ml, ab106777, Abcam, Cambridge, MA, USA), a mouse anti-human CD49d (ITGA4) antibody (1:100 dilution, 1 mg/ml, MCA697GA, AbD Serotec, Hercules, CA, USA), or normal mouse IgG (1:40 dilution, 0.4 mg/ml, sc-2025, Santa Cruz Biotechnology, Inc.) or normal rabbit IgG (1:80 dilution, 0.4 mg/ml, sc-2027, Santa Cruz Biotechnology, Inc.) as a Reproduction (2014) 148 119–127 A negative control.
Techniques: Expressing, Negative Control
Bio-Rad is a verified supplier 
